Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NMDAR2A (GluN2A) (extracellular)-ATTO-488 Antibody (#AGC-002-AG), (1:200) and Anti-SynGAP Antibody (#APZ-032), (1:200), followed by donkey-anti-rabbit-Cy3. A. GluN2A staining (green) appears in neuronal profiles (arrows). B. SynGAP staining (red) is detected mostly in apical dendrites (horizontal arrows) and in neuronal soma (vertical arrows). C. Merge of the two images demonstrates colocalization in some neurons (vertical arrows). Cell nuclei are stained with DAPI (blue).N-Methyl-D-Aspartic Acid
Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry. It has been designed to recognize GluN2A from rat, mouse, and human samples.
Anti-NMDAR2A (GluN2A) (extracellular)-ATTO-488 Antibody (#AGC-002-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-NMDAR2A (GluN2A) (extracellular)-ATTO-488 Antibody has been tested in immunohistochemistry and is especially suited for experiments requiring simultaneous labeling of different markers.
